Kobayashi T K, Okamoto H, Yakushiji M
Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation, Inc., Japan.
Diagn Cytopathol. 1993;9(3):296-9. doi: 10.1002/dc.2840090310.
Cytologic changes in the smears from nipple discharge of two cases with herpes simplex virus (HSV) infections are described. The cytology revealed the ground-glass appearance of the nuclei with multinucleated syncytial cells. Subsequently, in situ hybridization (ISH) using a biotinylated DNA probe was applied to identify the HSV DNA in the Papanicolaou-destained specimens. Positive hybridization was found with intense staining for the HSV DNA in the nuclei of cells having a ground-glass appearance. Cytologic observation together with ISH procedure may be rapid and valuable tool for the detection and final demonstration of HSV infections. Similar investigations may be carried out in cellular samples from other body sites.
描述了2例单纯疱疹病毒（HSV）感染患者乳头溢液涂片的细胞学变化。细胞学检查显示细胞核呈毛玻璃样外观，可见多核巨细胞。随后，应用生物素化DNA探针进行原位杂交（ISH），以鉴定巴氏染色标本中的HSV DNA。在细胞核呈毛玻璃样外观的细胞中发现了HSV DNA的阳性杂交信号，染色强烈。细胞学观察结合ISH方法可能是检测和最终证实HSV感染的快速且有价值的工具。类似的研究也可在来自身体其他部位的细胞样本中进行。
