Wong J Y, Zaharopoulos P, Van Dinh T
Acta Cytol. 1985 Sep-Oct;29(5):701-4.
Routine Papanicolaou-stained cervicovaginal smears from 59 patients were cytologically screened for herpetic infection. Forty-one of the smears were positive for herpes, 2 were suspicious and 16 were negative. All 59 slides were then destained and restained by a commercial immunoperoxidase kit for the detection of herpes simplex virus (HSV). The immunoperoxidase stain was positive in 23 of the 41 cytologically positive slides. One of the 2 cytologically suspicious slides was also immunoperoxidase positive, as was 1 of the 16 cytologically negative slides. This study indicates that immunoperoxidase staining is very specific but not quite as sensitive as routine Papanicolaou-stained smears in the detection of HSV. The immunoperoxidase method is thus recommended for the confirmation of HSV cases rather than for the routine diagnosis of HSV infection.
对59例患者的常规巴氏染色宫颈阴道涂片进行了细胞学检查，以筛查疱疹感染。其中41份涂片疱疹检测呈阳性，2份可疑，16份阴性。然后，对所有59张玻片进行脱色处理，并用商用免疫过氧化物酶试剂盒重新染色，以检测单纯疱疹病毒（HSV）。在41份细胞学阳性涂片中，免疫过氧化物酶染色有23份呈阳性。2份细胞学可疑涂片中的1份免疫过氧化物酶染色也呈阳性，16份细胞学阴性涂片中的1份同样呈阳性。本研究表明，免疫过氧化物酶染色特异性很强，但在检测HSV方面不如常规巴氏染色涂片敏感。因此，推荐使用免疫过氧化物酶法来确诊HSV病例，而非用于HSV感染的常规诊断。
