Gricourt L, Bonnec G, Boujard D, Mathieu M, Kellner K
Laboratoire de Biologie et Biotechnologies Marines, EA 962, UMR IFREMER Physiologie et Ecophysiologie des Mollusques Marins, Université de Caen Basse-Normandie, Caen Cedex 14032, France.
Gen Comp Endocrinol. 2003 Oct 15;134(1):44-56. doi: 10.1016/s0016-6480(03)00217-x.
The involvement of molecules belonging to the insulin/IGF family in regulation of growth has been investigated in the Pacific oyster Crassostrea gigas. In vitro biological effects of human recombinant IGF-1 (hrIGF-1) on mantle edge cells, involved in oyster shell and soft body growth, were studied over an annual cycle. In mantle edge cells hrIGF-1 stimulates protein synthesis of 56+/-5.1% over basal for 10(-10) M in September with in addition a clear dose-effect corresponding to the highest shell growth period, and 57.5+/-3.45% over basal for 10(-11) M in March and 51+/-5.4% over basal for 10(-10) M in April corresponding to the period of mantle growth. These insulin-like effects were associated with the expression of a recently identified C. gigas insulin receptor-related receptor (CIR) in mantle edge cells as demonstrated by RT-PCR. Moreover, in situ hybridisation (ISH) confirmed this expression at the level of the inner and outer epithelia involved in mantle growth and shell formation.
胰岛素/胰岛素样生长因子（IGF）家族分子在太平洋牡蛎（Crassostrea gigas）生长调控中的作用已得到研究。在一个年度周期内，研究了重组人胰岛素样生长因子-1（hrIGF-1）对参与牡蛎贝壳和软体生长的外套膜边缘细胞的体外生物学效应。在9月，10⁻¹⁰ M的hrIGF-1刺激外套膜边缘细胞的蛋白质合成比基础水平高出56±5.1%，此外，在贝壳生长最快的时期呈现明显的剂量效应；在3月，10⁻¹¹ M的hrIGF-1刺激蛋白质合成比基础水平高出57.5±3.45%；在4月，10⁻¹⁰ M的hrIGF-1刺激蛋白质合成比基础水平高出51±5.4%，这与外套膜生长时期相对应。这些胰岛素样效应与外套膜边缘细胞中最近鉴定出的太平洋牡蛎胰岛素受体相关受体（CIR）的表达相关，这通过逆转录聚合酶链反应（RT-PCR）得以证明。此外，原位杂交（ISH）在参与外套膜生长和贝壳形成的内、外上皮水平证实了这种表达。
