对暴露于升高的二氧化碳分压环境下的太平洋牡蛎（Crassostrea gigas）外套膜组织进行代谢组和蛋白质组联合分析。
Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2.
作者信息
Wei Lei, Wang Qing, Ning Xuanxuan, Mu Changkao, Wang Chunlin, Cao Ruiwen, Wu Huifeng, Cong Ming, Li Fei, Ji Chenglong, Zhao Jianmin
机构信息
Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.
出版信息
Comp Biochem Physiol Part D Genomics Proteomics. 2015 Mar;13:16-23. doi: 10.1016/j.cbd.2014.12.001. Epub 2014 Dec 20.
Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.
海洋酸化（OA）已被发现会影响软体动物的一系列正常生理过程，尤其对软体动物贝壳的形成过程构成重大威胁。在本研究中，采用整合代谢组学和蛋白质组学方法，研究了暴露于高pCO2条件下对太平洋牡蛎外套膜组织的影响。代谢组和蛋白质组分析表明，pCO2升高会影响太平洋牡蛎的能量代谢，其特征是ATP、琥珀酸、苹果酸脱氢酶、磷酸烯醇式丙酮酸羧激酶和醛脱氢酶水平发生差异变化。此外，钙调蛋白-2、原肌球蛋白和肌球蛋白轻链的上调表明，pCO2升高可能导致太平洋牡蛎外套膜组织的细胞骨架结构紊乱。这项工作表明，蛋白质组学和代谢组学相结合可以在分子水平上为海洋酸化的影响提供重要见解。
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