Stípek S, Jílek L
Physiol Bohemoslov. 1976;25(1):31-6.
In the rat prosencephalon it proved possible to differentiate lysosomal ribonuclease from alkaline ribonuclease activity, which could be detected only in the presence of p-chlormercuribenzoate. Acid RNase activity related to the amount of protein in the prosencephalon fell during ontogenesis. It was not significantly affected by four hours' stagnant hypoxia induced by ligation of both carotids. Its release from the lysosomes rose, however (when isotonic homogenates were spun at 20,000 g, acid ribonuclease activity in the supernatants was elevated). The absence of correlation between this activation and the degree of maturity of the nervous tissue refutes the hypothesis that regulation of this enzyme is per se responsible for the known changes induced by hypoxia in the RNA content of the prosencephalon of rats of different ages. On the contrary, the results indirectly support studies which demonstrate changes in the extent of RNA synthesis after hypoxia.
在大鼠前脑，已证实有可能区分溶酶体核糖核酸酶和碱性核糖核酸酶活性，后者只有在对氯汞苯甲酸存在时才能检测到。与前脑蛋白质含量相关的酸性核糖核酸酶活性在个体发育过程中下降。双侧颈动脉结扎诱导的4小时停滞性缺氧对其没有显著影响。然而，其从溶酶体中的释放增加（当等渗匀浆在20,000g下离心时，上清液中的酸性核糖核酸酶活性升高）。这种激活与神经组织成熟程度之间缺乏相关性，驳斥了关于该酶的调节本身导致不同年龄大鼠前脑缺氧诱导的已知RNA含量变化的假设。相反，这些结果间接支持了那些证明缺氧后RNA合成程度发生变化的研究。
