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与原胶原酶相比，基质溶解素在人关节软骨中的优先mRNA表达及原位定位。

Preferential mRNA expression of prostromelysin relative to procollagenase and in situ localization in human articular cartilage.

作者信息

Nguyen Q, Mort J S, Roughley P J

机构信息

Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.

出版信息

J Clin Invest. 1992 Apr;89(4):1189-97. doi: 10.1172/JCI115702.

DOI:10.1172/JCI115702
PMID:1313449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC442978/
Abstract

An imbalance between extracellular proteinases and their inhibitors is thought to underlie cartilage degradation. In cultures of adult cartilage, prostromelysin mRNA levels were much higher than those for procollagenase and this differential was increased in cultures stimulated with IL-1 beta. Analysis of mRNA prepared from freshly isolated chondrocytes showed abundant amounts of prostromelysin mRNA in normal adult cartilage but low levels in the neonate. Not all adult cartilage may possess such high levels of prostromelysin mRNA, as the message levels in the cartilage remaining on late-stage osteoarthritic joints were lower than those in normal adult cartilage. Relative to prostromelysin mRNA, little procollagenase and TIMP mRNA were found in the adult cartilage. In situ hybridization revealed that metalloproteinase mRNAs were localized in chondrocytes of the superficial zone in adult cartilage. However, upon IL-1 beta treatment, chondrocytes in all cartilage zones were observed to express prostromelysin mRNA. Relative to the neonate, the normal adult cartilage appears to have a high degradative potential, if one accepts that steady-state mRNA levels reflect prostromelysin production. As the adult cartilage is not apparently undergoing rapid turnover, it would appear that control of prostromelysin activation may be the major regulatory step in stromelysin-induced cartilage degradation.

摘要

细胞外蛋白酶与其抑制剂之间的失衡被认为是软骨降解的基础。在成年软骨培养物中，前基质溶解素mRNA水平远高于前胶原酶，并且在用IL-1β刺激的培养物中这种差异会增加。对新鲜分离的软骨细胞制备的mRNA分析表明，正常成年软骨中存在大量的前基质溶解素mRNA，但新生儿软骨中的水平较低。并非所有成年软骨都可能具有如此高水平的前基质溶解素mRNA，因为晚期骨关节炎关节残留软骨中的信使水平低于正常成年软骨。相对于前基质溶解素mRNA，在成年软骨中发现的前胶原酶和TIMP mRNA很少。原位杂交显示金属蛋白酶mRNA定位于成年软骨表层区的软骨细胞中。然而，在用IL-1β处理后，观察到所有软骨区的软骨细胞都表达前基质溶解素mRNA。相对于新生儿，如果认为稳态mRNA水平反映前基质溶解素的产生，那么正常成年软骨似乎具有较高的降解潜力。由于成年软骨显然没有经历快速更新，因此前基质溶解素激活的控制似乎是基质溶解素诱导的软骨降解中的主要调节步骤。

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