Van Der Maaten M J, Whetstone C A
Virology Cattle Research Unit, National Animal Disease Center, USDA, Ames, IA 50010.
Vet Microbiol. 1992 Feb;30(2-3):125-35. doi: 10.1016/0378-1135(92)90108-6.
New Zealand white rabbits, which had been prepared for inoculation by intraperitoneal treatment with thioglycollate, were inoculated intraperitoneally with bovine immunodeficiency-like virus (BIV). Infected materials from various sources were used including cultured cells and culture fluids, peripheral blood leukocytes from infected cattle and spleen tissue from previously infected rabbits. Virus isolations and serological responses detected by western blotting provided clear evidence that infections had been established in inoculated rabbits and that the spleen was an important site of BIV infectivity. These results indicate that rabbits may be a useful species when testing for BIV infectivity in materials too toxic or highly contaminated to be inoculated directly into cell cultures. Furthermore, rabbits may also be useful in testing effects of coinfections with other bovine viruses on progression of BIV infection and for the initial evaluation of therapeutic regimens designed to suppress or eliminate BIV infections.
通过腹腔注射巯基乙酸盐进行接种准备的新西兰白兔，经腹腔接种牛免疫缺陷样病毒（BIV）。使用了来自各种来源的感染材料，包括培养细胞和培养液、感染牛的外周血白细胞以及先前感染兔子的脾脏组织。通过病毒分离和蛋白质印迹法检测到的血清学反应提供了明确的证据，表明接种的兔子已建立感染，且脾脏是BIV感染的重要部位。这些结果表明，在检测毒性过大或污染严重而无法直接接种到细胞培养物中的材料的BIV感染性时，兔子可能是一种有用的动物。此外，兔子在测试与其他牛病毒的共感染对BIV感染进展的影响以及对旨在抑制或消除BIV感染的治疗方案进行初步评估方面也可能有用。
