A cellular-biochemical evaluation of gastric body mucosa and muscular layer in patients with different basal acid outputs.

The membrane (Na+-K+ -dependent) ATP-ase (EC 3.6.1.3.), adenine-adenosine, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP), phospholipid phosphates (lipid-P), ribonucleic acid (RNA), and deoxyribonucleic acid (DNA) were extracted and measured in the patients' gastric body mucosa and muscular layer with different basal acid outputs. It was observed that the Na+-K+ -dependent ATP-ase activity, tissue adenine adenosine, AMP, ADP, ATP, lipid-P, and RNA - in correspondence to 1.0 mg DNA - significantly increased in the gastric corpus mucosa, with an increase of basal acid output. No significant changes of these parameters were found to be present in the gastric body muscular layer of patients with different basal acid output. It has been concluded: 1) the energy requirement for H+ secretion, from the point of view of basal acid output, is given by the transformation of ATP into ADP; 2) the biochemical building up of the human gastric mucosa is different in patients with decreased, normal and increased BAO values; 3) no significant difference is found between the biochemical structures of gastric muscular layer in patients with different basal acid output values.