Berg C M, Vartak N B, Wang G, Xu X, Liu L, MacNeil D J, Gewain K M, Wiater L A, Berg D E
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
Gene. 1992 Apr 1;113(1):9-16. doi: 10.1016/0378-1119(92)90664-b.
Transposon gamma delta (Tn1000), a 6-kb member of the Tn3 family, is widely used for plasmid mutagenesis. A 1.8-kb derivative of gamma delta was constructed that contains the kan gene from Tn5 and the resolution (res) site from gamma delta cloned between 40-bp inverted repeats of gamma delta's delta (delta) end. This element, named m gamma delta-1, lacks the genes encoding transposase and resolvase, and therefore depends on its host to supply transposition and resolution functions. Thus, in strains lacking gamma delta, m gamma delta-1 will not transpose. The m gamma delta-1 element is shown to be useful for mutagenesis of plasmids, DNA sequencing, and allele replacement (in Streptomyces avermitilis).
转座子γδ（Tn1000）是Tn3家族的一个6kb成员，广泛用于质粒诱变。构建了γδ的一个1.8kb衍生物，它包含来自Tn5的卡那霉素基因（kan基因）和来自γδ的解离位点（res位点），克隆在γδ的δ末端的40bp反向重复序列之间。这个元件名为mγδ-1，缺少编码转座酶和解离酶的基因，因此依赖其宿主提供转座和解离功能。因此，在缺乏γδ的菌株中，mγδ-1不会发生转座。mγδ-1元件已被证明可用于质粒诱变、DNA测序和等位基因替换（在阿维链霉菌中）。
