Zaffaroni N, Villa R, Orlandi L, Vaglini M, Silvestrini R
Oncologia Sperimentale C, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
Anticancer Res. 1992 Jan-Feb;12(1):247-50.
The in vitro cytotoxic activity of a 1 hr-treatment with lonidamine alone or combined with hyperthermia was investigated on primary cultures obtained from 12 human malignant melanomas and 9 lung carcinomas. The study was carried out by an antiproliferative assay based upon the inhibition of incorporation of 3H-thymidine into DNA of cells grown in agarose for 4 days. Under normothermic conditions the drug did not have any cytotoxic effect on either tumor type. Hyperthermia alone also failed to influence proliferation of melanoma and lung carcinoma cells. When the same tumors were exposed to lonidamine at 42 degrees C, there was significant enhancement of drug activity in both tumor types. There was also a synergistic interaction in a percentage of tumors, which increased as a function of drug concentration.
研究了单独使用氯尼达明或与热疗联合进行1小时处理对取自12例人类恶性黑色素瘤和9例肺癌的原代培养物的体外细胞毒性活性。该研究通过基于抑制3H-胸腺嘧啶核苷掺入在琼脂糖中生长4天的细胞DNA的抗增殖试验来进行。在常温条件下，该药物对这两种肿瘤类型均无任何细胞毒性作用。单独的热疗也未能影响黑色素瘤和肺癌细胞的增殖。当相同的肿瘤在42℃下暴露于氯尼达明时，两种肿瘤类型的药物活性均有显著增强。在一定比例的肿瘤中还存在协同相互作用，且该比例随药物浓度增加而增加。
