Zaffaroni N, Villa R, Daidone M G, Vaglini M, Santinami M, Silvestrini R
Divisione di Oncologia Sperimentale C, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Int J Cell Cloning. 1989 Nov;7(6):385-94. doi: 10.1002/stem.5530070606.
The effects of heat and the interaction between hyperthermia and alkylating agents, such as cisplatin (CDDP) and melphalan (L-PAM) in human malignant melanoma biopsies have been investigated by a short-term assay based upon the inhibition of 3H-thymidine incorporation. Cell suspensions from 50 cutaneous and lymph nodal metastases were heated at 40.5 degrees C or at 42 degrees C for 1 h. There were significant antiproliferative effects due to heat in 10% of the tumors exposed to 40.5 degrees C and 34% to 42 degrees C. Thermal resistance was evident in 73% (at 40.5 degrees C) and 54% (at 43 degrees C) of tumors, and there was significant enhancement of cell growth in 17% and 12% of tumors. The combined effects of hyperthermia and drugs were studied on 36 tumors. Cell suspensions were exposed to different concentrations of CDDP or L-PAM for 1 h at 40.5 degrees C and 42 degrees C. Synergy between heat and CDDP was observed in 7% of cases treated with the lowest drug dose and 38% of cases treated with the highest (40.5 degrees C), with only a slight increase in the frequency of synergy at 42 degrees C. Synergy between heat and L-PAM was also observed in 12% to 44% of tumors at 42 degrees C as a function of drug concentration.
通过基于抑制³H-胸腺嘧啶核苷掺入的短期试验，研究了热以及热疗与顺铂（CDDP）和美法仑（L-PAM）等烷化剂在人恶性黑色素瘤活检组织中的相互作用。来自50例皮肤和淋巴结转移灶的细胞悬液在40.5℃或42℃加热1小时。在暴露于40.5℃的肿瘤中，10%因热产生了显著的抗增殖作用；在暴露于42℃的肿瘤中，34%产生了显著的抗增殖作用。73%（40.5℃时）和54%（43℃时）的肿瘤表现出耐热性，17%和12%的肿瘤细胞生长显著增强。对36个肿瘤研究了热疗与药物的联合作用。细胞悬液在40.5℃和42℃下暴露于不同浓度的CDDP或L-PAM 1小时。在使用最低药物剂量治疗的病例中，7%观察到热与CDDP之间的协同作用；在使用最高药物剂量治疗的病例中，38%观察到热与CDDP之间的协同作用（40.5℃时），在42℃时协同作用频率仅略有增加。在42℃时，热与L-PAM之间的协同作用也在12%至44%的肿瘤中观察到，这是药物浓度的函数。
