Brahem A, Demarquez N, Beyrie M, Vuillaume A, Fleury H J
Laboratoire Départemental des Landes, Mont de Marsan, France.
Avian Dis. 1992 Jan-Mar;36(1):143-8.
During a 1986 natural lethal outbreak of fulminating disease in guinea poult flocks in southwestern France, enveloped virus particles were consistently observed in the gut contents of infected birds. For the present study, a protocol was developed for the purification of these particles. Sucrose-banded virus obtained from birds infected experimentally with virus from the outbreak was found to have a buoyant density of 1.18 g/ml. The purified virus showed hemagglutinating activity, was shown by electron microscopy to have a togavirus-like morphology, and also was shown to be transmissible and pathogenic through oral ingestion. In addition, other enveloped particles have been occasionally detected in gut contents of both infected and uninfected birds; the improbability of the viral nature of these interfering particles is discussed.
1986年，法国西南部的珍珠鸡群爆发了一场自然致死性暴发性疾病，在受感染禽类的肠道内容物中持续观察到有包膜的病毒颗粒。在本研究中，制定了一种纯化这些颗粒的方案。从实验感染了此次疫情病毒的禽类中获得的蔗糖梯度离心病毒，其浮力密度为1.18 g/ml。纯化后的病毒表现出血凝活性，电子显微镜显示其具有披膜病毒样形态，并且通过口服摄入具有传染性和致病性。此外，在受感染和未受感染禽类的肠道内容物中偶尔也检测到其他有包膜颗粒；讨论了这些干扰颗粒具有病毒性质的可能性。
