Kimman T G, Brouwers R A, Daus F J, van Oirschot J T, van Zaane D
Central Veterinary Institute, Department of Virology, Lelystad, Netherlands.
Vet Immunol Immunopathol. 1992 Feb 15;31(1-2):95-113. doi: 10.1016/0165-2427(92)90089-9.
Enzyme-linked immunosorbent assays (ELISAs) for the detection of porcine IgM, IgA, IgG1 and IgG2 antibodies directed against Aujeszky's disease virus (ADV) are described. ADV-specific IgA and IgM were detected in an antibody capture assay, and ADV-specific IgG1 and IgG2 were detected in an indirect double antibody sandwich assay. A selected set of samples was tested in the four ELISAs and in a 24 h virus neutralization assay. Comparison of the results showed that the ELISAs were isotype-specific, sensitive, and reproducible. Samples with ADV antibody of one isotype showed that ADV-specific IgG1, IgG2 and IgM were able to neutralize the virus in vitro. In vitro neutralization of virus can be enhanced by complement. ADV-specific IgA neutralized virus only weakly. ADV-infected cells activated complement in the absence of antibody. Specific IgG2 and IgM enhanced complement activation. Analysis of the time course of antibody responses after infection or vaccination revealed that the isotype-specific ELISAs are suitable to study the humoral antibody response of pigs to the virus in mucosal secretions. Wild-type virus (strain NIA-3) and an attenuated vaccine strain (Bartha) administered intranasally induced mucosal IgM and IgA responses to the virus. In contrast, a killed vaccine (Nobivac) administered intramuscularly induced only weak mucosal IgM responses. The attenuated vaccine strain primed for a mucosal IgA memory response evoked upon challenge infection with wild-type virus.
本文描述了用于检测猪针对伪狂犬病病毒（ADV）的IgM、IgA、IgG1和IgG2抗体的酶联免疫吸附测定（ELISA）。在抗体捕获测定中检测到ADV特异性IgA和IgM，在间接双抗体夹心测定中检测到ADV特异性IgG1和IgG2。在四种ELISA和24小时病毒中和试验中对一组选定的样品进行了检测。结果比较表明，ELISA具有亚型特异性、敏感性和可重复性。具有一种亚型ADV抗体的样品显示，ADV特异性IgG1、IgG2和IgM能够在体外中和病毒。补体可增强病毒的体外中和作用。ADV特异性IgA仅能微弱地中和病毒。ADV感染的细胞在无抗体的情况下激活补体。特异性IgG2和IgM增强补体激活。对感染或接种疫苗后抗体反应的时间进程分析表明，亚型特异性ELISA适用于研究猪对病毒在粘膜分泌物中的体液抗体反应。经鼻接种野生型病毒（NIA-3株）和减毒疫苗株（Bartha）可诱导对该病毒的粘膜IgM和IgA反应。相比之下，肌肉注射灭活疫苗（Nobivac）仅诱导微弱的粘膜IgM反应。减毒疫苗株可引发针对野生型病毒攻击感染时诱发的粘膜IgA记忆反应。
