Comparison of an EBV transformed cell line and an EBV hybridoma cell line producing the same human anti-HBs monoclonal antibody.

作者信息

Sa'adu A, Walker H, Locniskar M, Bidwell D, Howard C, McAdam K P, Voller A

机构信息

Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.

出版信息

J Virol Methods. 1992 Apr;37(1):1-11. doi: 10.1016/0166-0934(92)90016-7.

A stable human-mouse heterohybridoma secreting human anti-HBs monoclonal antibody in continuous culture for 12 months was generated. It grew faster than the parent EBV transformed lymphoblastoid cell line (LCL) but produced the same level of specific antibody. The LCL was positive for the Epstein-Barr Virus Nuclear Antigen (EBNA), human CD 23 and contained a diploid number of human chromosomes. The heterohybridoma was negative for EBNA, CD 23 and mouse Ly-1 mouse, despite retaining a full complement of diploid mouse chromosomes and a limited number of human chromosomes.