使用酶联免疫吸附测定法筛选抗乙型肝炎表面抗原的杂交瘤抗体。
Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen.
作者信息
Boniolo A, Dovis M, Matteja R
出版信息
J Immunol Methods. 1982;49(1):1-15. doi: 10.1016/0022-1759(82)90361-1.
An enzyme-linked immunosorbent assay (ELISA) was developed for screening production of monoclonal antibodies with specificity for HBsAg. Mouse hybridoma IgG were firstly extracted from assay medium with goat anti-mouse IgG adsorbed on polystyrene beads. The specific antibody was revealed by saturation with HBs antigen from human positive sera followed by reaction with specific sheep anti-HBs antibody conjugated with peroxidase. The sensitivity was of the order of 0.5-1 ng/ml specific antibody and specificity was satisfactory. The assay permits easy identification of determinant specificity on screening.
开发了一种酶联免疫吸附测定法（ELISA），用于筛选对乙肝表面抗原（HBsAg）具有特异性的单克隆抗体的产生。首先用吸附在聚苯乙烯珠上的山羊抗小鼠IgG从测定培养基中提取小鼠杂交瘤IgG。通过用人阳性血清中的HBs抗原饱和，然后与与过氧化物酶偶联的特异性羊抗HBs抗体反应来揭示特异性抗体。灵敏度约为0.5 - 1 ng/ml特异性抗体，特异性令人满意。该测定法便于在筛选时鉴定决定簇特异性。
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