Dickson S R, Warburton M J
Department of Histopathology, St George's Hospital Medical School, London, United Kingdom.
J Histochem Cytochem. 1992 May;40(5):697-703. doi: 10.1177/40.5.1315355.
During the involution of the mammary gland there is destruction of the basement membrane as the secretory alveolar structures degenerate. Immunofluorescence staining of sections of rat mammary gland with antibodies to 72 KD gelatinase (MMP-2) and stromelysin (MMP-3) revealed increased production of these two proteinases during involution. This increased expression was mostly restricted to myoepithelial cells. Increased expression during involution was also demonstrated by immunoblotting techniques. Gelatin zymography indicated that the predominant metalloproteinase present in involuting rat mammary glands was a 66 KD gelatinase.
在乳腺退化过程中，随着分泌性肺泡结构的退化，基底膜会遭到破坏。用抗72 KD明胶酶（基质金属蛋白酶-2，MMP-2）和基质溶解素（基质金属蛋白酶-3，MMP-3）的抗体对大鼠乳腺切片进行免疫荧光染色，结果显示在退化过程中这两种蛋白酶的产生增加。这种表达增加主要局限于肌上皮细胞。免疫印迹技术也证实了退化过程中表达增加。明胶酶谱分析表明，退化的大鼠乳腺中存在的主要金属蛋白酶是一种66 KD明胶酶。
