Hishikawa K, Nakaki T, Tsuda M, Esumi H, Ohshima H, Suzuki H, Saruta T, Kato R
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Jpn Heart J. 1992 Jan;33(1):41-8. doi: 10.1536/ihj.33.41.
The effects of L-arginine administration on systemic hemodynamics and plasma concentrations of neuro-endocrine hormones and amino acids were investigated in 10 normotensive healthy volunteers. Nitrite/nitrate in urine and cyclic guanosine monophosphate (c-GMP) in plasma were also measured as indicators of release of nitric oxide (NO). L-arginine administration (30 g/300 ml/30 min) caused hypotension (mean arterial pressure; 79.3 +/- 3.9 mmHg fell to 68.8 +/- 2.2 mmHg) with tachycardia (62.3 +/- 2.3 beats/min increased to 67.5 +/- 1.9 beats/min). The plasma concentration of L-arginine before administration was 98.8 +/- 8.2 mumol/l and increased to 7263 +/- 567 mumol/l 20 min after administration. Cardiac output also increased to 127.2 +/- 3.9% by L-arginine administration. Total peripheral resistance was calculated to fall to 65.9 +/- 2.0%. L-arginine administration slightly changed several hormones, but all values were within normal ranges. Nitrite/nitrate in urine increased 142.1 +/- 12.4% compared to the values before L-arginine administration. Plasma concentrations of c-GMP and L-citrulline, the by-product of NO from L-arginine, were also significantly increased by L-arginine administration. All our results provide evidence for the first time that systemically administered L-arginine releases NO in man.
在10名血压正常的健康志愿者中研究了给予L-精氨酸对全身血流动力学、神经内分泌激素和氨基酸血浆浓度的影响。还测量了尿中亚硝酸盐/硝酸盐和血浆中环磷酸鸟苷（c-GMP）作为一氧化氮（NO）释放的指标。给予L-精氨酸（30 g/300 ml/30分钟）导致低血压（平均动脉压；从79.3±3.9 mmHg降至68.8±2.2 mmHg）并伴有心动过速（从62.3±2.3次/分钟增加到67.5±1.9次/分钟）。给药前L-精氨酸的血浆浓度为98.8±8.2 μmol/L，给药后20分钟增加到7263±567 μmol/L。给予L-精氨酸后心输出量也增加到127.2±3.9%。计算得出总外周阻力降至65.9±2.0%。给予L-精氨酸使几种激素略有变化，但所有值均在正常范围内。与给予L-精氨酸前的值相比，尿中亚硝酸盐/硝酸盐增加了142.1±12.4%。给予L-精氨酸后，血浆中c-GMP和L-瓜氨酸（L-精氨酸产生NO的副产物）的浓度也显著增加。我们所有的结果首次提供了证据，证明全身给予L-精氨酸可在人体内释放NO。
