Weber B, Harms F, Selb B, Liang X Y, Doerr H W
Abteilung für Medizinische Virologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt a.M.
Zentralbl Hyg Umweltmed. 1992 Mar;192(6):534-43.
In the present study, virus isolation was attempted in one hundred and twenty-one fecal samples of children suffering from acute gastroenteritis. Virus isolation was performed either conventionally by examination of cytopathogenic effect (CPE) or by immunoperoxidase staining (IPS) of rotavirus group specific antigen (inner capsid) in trypsin free MA104 cells within 18 h. Applying the conventional technique, rotavirus was isolated in only 4 (3.3%) fecal specimens. In contrast, IPS detected infectious virus in 49 (40.5%) stool samples. The specificity of IPS was confirmed by the results obtained with an antigen detection ELISA ("Rotazyme", Abbott, Wiesbaden) and gel electrophoresis of rotaviral RNA (electrophoretyping). ELISA and RNA gel electrophoresis detected rotavirus in 66 (54.4%) and 56 (46.3%) stool samples respectively. IPS enables rapid diagnosis of rotavirus infections in cell cultures applied to detect infectious viral particles in order to be used in the investigation of nosocomial outbreaks, material- and surface contamination and evaluation of disinfectants.
在本研究中，对121例患急性肠胃炎儿童的粪便样本进行了病毒分离。病毒分离采用常规方法，即通过检查细胞病变效应（CPE），或在18小时内在无胰蛋白酶的MA104细胞中对轮状病毒群特异性抗原（内衣壳）进行免疫过氧化物酶染色（IPS）。采用传统技术，仅在4份（3.3%）粪便标本中分离出轮状病毒。相比之下，IPS在49份（40.5%）粪便样本中检测到感染性病毒。通过抗原检测ELISA（“Rotazyme”，雅培公司，威斯巴登）和轮状病毒RNA凝胶电泳（电泳分型）获得的结果证实了IPS的特异性。ELISA和RNA凝胶电泳分别在66份（54.4%）和56份（46.3%）粪便样本中检测到轮状病毒。IPS能够在用于检测感染性病毒颗粒的细胞培养物中快速诊断轮状病毒感染，以便用于医院感染暴发调查、材料和表面污染检测以及消毒剂评估。
