Weber B, Harms F, Selb B, Doerr H W
Abteilung für Medizinische Virologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt a.M., Germany.
J Virol Methods. 1992 Aug;38(2):187-94. doi: 10.1016/0166-0934(92)90109-q.
Peroxidase-labeled monoclonal antibody against rotavirus group-specific antigen (inner capsid) was used for the detection of rotavirus by immunoperoxidase staining (IPS) in trypsin-free MA104 cells within 18 h post-inoculation with clinical specimens. One hundred and twenty-one fecal samples from children with acute gastroenteritis were evaluated by IPS, conventional virus isolation in cell culture and a commercially available group A-antigen ELISA (Rotazyme II, Abbott Laboratories). Fifty-eight (47.9%) stool samples were found positive by IPS. In contrast, rotavirus was isolated from only 4 (3.3%) fecal specimens by conventional cell culture (i.e. demonstration of a cytopathogenic effect). A total of 93 (76.9%) samples were positive by ELISA. IPS permits rapid detection of rotavirus infections and detects shedding of infectious virus. The method should be useful for the investigation of nosocomial spread of rotavirus infection in hospitals, contamination of environmental surfaces and desinfectants.
用抗轮状病毒组特异性抗原（内衣壳）的过氧化物酶标记单克隆抗体，通过免疫过氧化物酶染色（IPS），在接种临床标本后18小时内，于无胰蛋白酶的MA104细胞中检测轮状病毒。采用IPS、常规细胞培养病毒分离法以及市售的A组抗原酶联免疫吸附试验（Rotazyme II，雅培实验室），对121份急性胃肠炎患儿的粪便样本进行了评估。IPS检测发现58份（47.9%）粪便样本呈阳性。相比之下，通过常规细胞培养（即细胞病变效应的证明）仅从4份（3.3%）粪便标本中分离出轮状病毒。酶联免疫吸附试验显示共有93份（76.9%）样本呈阳性。IPS可快速检测轮状病毒感染，并检测传染性病毒的排出。该方法对于调查医院内轮状病毒感染的传播、环境表面污染及消毒剂效果应是有用的。
