乳胶凝集试验、聚丙烯酰胺凝胶电泳与酶联免疫吸附测定法在粪便标本中检测人轮状病毒的比较。
Comparison of latex agglutination and polyacrylamide gel electrophoresis with enzyme linked immunosorbent assay for detecting human rotavirus in stool specimens.
作者信息
Chakravarti A, Kumar S, Mittal S K, Broor S
机构信息
Department of Microbiology, Maulana Azad Medical College, New Delhi.
出版信息
Indian Pediatr. 1991 May;28(5):507-10.
One hundred and forty five stool samples from children below 2 years of age, hospitalized with diarrhea were tested for rotavirus antigen by enzyme linked immunosorbent assay (ELISA), latex agglutination test using commercially available kit Rotastat (Ranbaxy Diagnostic, India) and by polyacrylamide gel electrophoresis. Twenty eight samples were positive for the virus antigen by all the three assay systems. The sensitivity of latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE) was 91.4% (32/35) and 80% (28/35), respectively; the corresponding specificity was 98.18% (108/110) and 100% (110/110), respectively. Latex agglutination was the least complex, required the least amount of apparatus and provided a result within a short time. It showed a high specificity and a reasonable amount of sensitivity and the results correlated well with ELISA and PAGE.
对145例2岁以下因腹泻住院儿童的粪便样本进行检测，采用酶联免疫吸附测定（ELISA）、使用市售Rotastat试剂盒（印度兰伯西诊断公司）的乳胶凝集试验以及聚丙烯酰胺凝胶电泳检测轮状病毒抗原。所有三种检测系统均显示28份样本的病毒抗原呈阳性。乳胶凝集试验（LA）和聚丙烯酰胺凝胶电泳（PAGE）的灵敏度分别为91.4%（32/35）和80%（28/35）；相应的特异性分别为98.18%（108/110）和100%（110/110）。乳胶凝集试验最为简便，所需仪器最少，且能在短时间内得出结果。它具有高特异性和合理的灵敏度，结果与ELISA和PAGE相关性良好。
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