Miyakoshi S, Haruyama H, Shioiri T, Takahashi S, Torikata A, Yamazaki M
Fermentation Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
J Antibiot (Tokyo). 1992 Mar;45(3):394-9. doi: 10.7164/antibiotics.45.394.
Biosynthesis of griseolic acids, competitive inhibitors of cyclic nucleotide phosphodiesterase, was investigated with the culture of a producing strain of Streptomyces griseoaurantiacus. 13C-Labeled and 15N-labeled compounds were added into the culture, and 13C-enriched and 15N-enriched griseolic acid A was isolated from the culture medium and analyzed by 13C NMR and 15N NMR spectroscopy. The compounds added to growth medium were [2-13C]acetate, [1,2-13C]acetate, [1,4-13C]succinate, [1-13C]glucose, [6-13C]glucose, [2-13C]ribose, and [1-13C, 15N]glycine. The results suggest that adenosine, which is formed from amino acids and sugars contributes the adenine and ribose moieties to griseolic acid A. The data also suggest that a dicarboxylic acid from the Krebs tricarboxylic acid cycle contributes to the dicarboxylic part of the compound.
研究了环核苷酸磷酸二酯酶竞争性抑制剂灰黄霉素酸的生物合成，以灰黄橙链霉菌生产菌株进行培养。向培养物中添加了13C标记和15N标记的化合物，从培养基中分离出富含13C和15N的灰黄霉素酸A，并通过13C NMR和15N NMR光谱进行分析。添加到生长培养基中的化合物有[2-13C]乙酸盐、[1,2-13C]乙酸盐、[1,4-13C]琥珀酸盐、[1-13C]葡萄糖、[6-13C]葡萄糖、[2-13C]核糖和[1-13C, 15N]甘氨酸。结果表明，由氨基酸和糖类形成的腺苷为灰黄霉素酸A提供了腺嘌呤和核糖部分。数据还表明，来自三羧酸循环的二羧酸为该化合物的二羧酸部分做出了贡献。
