Gray H W, Tsan M F, Wagner H N
J Nucl Med. 1977 Feb;18(2):147-50.
Leukocyte cell-to-cell adhesion was studied with a cell monolayer technique. The amount of protein retained on the coverslip reflected the number of adhering cells. When polymorphonuclear leukocytes were suspended in a balanced salt solution at neutral pH containing no bivalent cations, they adhered to the glass surface but not to each other. Magnesium or calcium at concentrations above 1 mM caused cell-to-cell adhesion. Stannous and chromate ions had no effect on leukocyte cell-to-cell adhesion. Acid pH also induced leukocyte aggregation. This last effect could be reversed by washing the cells with a balanced salt solution at neutral pH, but it could not be reversed by alcohol.
采用细胞单层技术研究白细胞间的细胞黏附。盖玻片上保留的蛋白量反映了黏附细胞的数量。当中性pH值的平衡盐溶液中不含二价阳离子时，多形核白细胞悬浮其中，它们会黏附于玻璃表面，但不会相互黏附。浓度高于1 mM的镁离子或钙离子会导致细胞间黏附。亚锡离子和铬酸根离子对白细胞间的细胞黏附没有影响。酸性pH值也会诱导白细胞聚集。最后这种效应可通过用中性pH值的平衡盐溶液洗涤细胞来逆转，但不能通过酒精来逆转。
