Doly M, Chassagne J, Demeocq F, Finat-Duclos F, Doly A, Plagne R, Veyre A, Besse G, Gaillard G, Meyniel G
Eur J Nucl Med. 1982;7(9):397-404. doi: 10.1007/BF00256818.
An original 99mTc-labeling method applied to human lymphocytes is described. This technique is based on the use of stannous pyrophosphate to reduce sodium pertechnetate. The proposed procedure has two main advantages: firstly, the labeling process takes place in a neutral and buffered medium which prevents any cellular aggregation; secondly, the labeling yield obtained is compatible with scintigraphic studies in man. The influence of each parameter on the labeling quality had been studied as well as the binding stability of the technetium fixed on cells. A systematic study of lymphocyte surface markers, before and after labeling, has led to the suggestion that the labeling process seems to affect cellular membranes, probably because of the technetium binding. Finally, scintigraphic results are presented, the study being performed on eight healthy volunteers. These results are compared with those published by other authors who used either a different radioisotope or a different labeling method.
本文描述了一种应用于人类淋巴细胞的原始99mTc标记方法。该技术基于使用焦磷酸亚锡还原高锝酸钠。所提出的程序有两个主要优点：首先，标记过程在中性缓冲介质中进行，可防止任何细胞聚集；其次，获得的标记产率与人体闪烁扫描研究兼容。研究了每个参数对标记质量的影响以及固定在细胞上的锝的结合稳定性。对标记前后淋巴细胞表面标志物的系统研究表明，标记过程似乎会影响细胞膜，可能是由于锝的结合。最后，给出了闪烁扫描结果，该研究是在八名健康志愿者身上进行的。这些结果与其他使用不同放射性同位素或不同标记方法的作者发表的结果进行了比较。
