Tominaga K, Shibata S, Ueki S, Watanabe S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Eur J Pharmacol. 1992 Apr 7;214(1):79-84. doi: 10.1016/0014-2999(92)90099-p.
The effects of 5-HT1A receptor agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), buspirone and ipsapirone on wheel-running activity in hamsters were investigated in comparison with those of GABAA receptor agonist muscimol and benzodiazepine triazolam. Intraperitoneal administration of 8-OH-DPAT, buspirone, ipsapirone, muscimol and triazolam at circadian time (CT) 8 (CT 12; onset of activity) induced a significant phase advance of wheel-running activity under constant light conditions. However, administration of these drugs at other CT points did not induce phase changes. The administration of trifluoromethylphenylpiperazine (TFMPP), a 5-HT1B receptor agonist, at CT8 produced a small phase advance. The phase advance induced by 8-OH-DPAT was blocked by pretreatment with (-)-pindolol, a 5-HT1A receptor antagonist. In addition, 8-OH-DPAT, buspirone and SM3997 accelerated the rate of re-entrainment to an 8-h phase advance in the light-dark cycle. These observations suggest that 5-HT1A receptors in the brain participate in the regulation of the circadian rhythm of wheel-running activity in hamsters.
将5-羟色胺1A受体激动剂8-羟基-2-（二正丙基氨基）四氢萘（8-OH-DPAT）、丁螺环酮和伊沙匹隆对仓鼠转轮活动的影响，与γ-氨基丁酸A型（GABAA）受体激动剂蝇蕈醇和苯二氮䓬类三唑仑的影响进行了比较研究。在昼夜时间（CT）8（CT 12；活动开始）腹腔注射8-OH-DPAT、丁螺环酮、伊沙匹隆、蝇蕈醇和三唑仑，在持续光照条件下可诱导转轮活动出现显著的相位提前。然而，在其他CT时间点给予这些药物并未诱导相位变化。在CT8给予5-羟色胺1B受体激动剂三氟甲基苯基哌嗪（TFMPP）可产生较小的相位提前。8-OH-DPAT诱导的相位提前可被5-羟色胺1A受体拮抗剂（-）-吲哚洛尔预处理所阻断。此外，8-OH-DPAT、丁螺环酮和SM3997加快了在明暗周期中重新适应提前8小时相位的速率。这些观察结果表明，脑中的5-羟色胺1A受体参与了仓鼠转轮活动昼夜节律的调节。
