Goessling L S, Daniels-McQueen S, Bhattacharyya-Pakrasi M, Lin J J, Thach R E
Department of Biology, Washington University, St. Louis, MO 63130.
Science. 1992 May 1;256(5057):670-3. doi: 10.1126/science.1316633.
Induction of ferritin synthesis in cultured cells by heme or iron is accompanied by degradation of the ferritin repressor protein (FRP). Intermediates in the degradative pathway apparently include FRP covalently linked in larger aggregates. The effect of iron on FRP degradation is enhanced by porphyrin precursors but is decreased by inhibitors of porphyrin synthesis, which implies that heme is an active agent. These results suggest that translational induction in this system may be caused by enhanced repressor degradation. While unique among translational regulatory systems, this process is common to a variety of other biosynthetic control mechanisms.
血红素或铁在培养细胞中诱导铁蛋白合成时，伴随着铁蛋白阻遏蛋白（FRP）的降解。降解途径中的中间体显然包括以较大聚集体形式共价连接的FRP。卟啉前体可增强铁对FRP降解的作用，但卟啉合成抑制剂可降低该作用，这表明血红素是一种活性剂。这些结果表明，该系统中的翻译诱导可能是由阻遏物降解增强所致。虽然这在翻译调控系统中是独特的，但这一过程在多种其他生物合成控制机制中很常见。
