Deregt D, Smithson S, Kozub G C
Animal Diseases Research Institute, Lethbridge, Alberta.
Can J Vet Res. 1992 Apr;56(2):161-4.
A three day serum neutralization (SN) test for the detection of antibodies to bovine viral diarrhea virus (BVDV), which is an improvement on the existing five day test, is described. The improved test results in a more rapid viral cytopathic effect and utilizes Madin Darby kidney (MDBK) cells, and horse serum as a medium supplement. A comparison of tests utilizing the NADL and the Singer strains of BVDV and the use of either secondary bovine kidney cells with calf serum (BKCS) or continuous MDBK cells with horse serum (MDHS) was performed. Analysis of the SN results of 685 serum samples from 445 Quebec and Ontario cattle showed that there was no difference, as expected, in the means of the SN antibody titers when the NADL strain was used in either the BKCS or MDHS system but SN antibody titers were elevated (p less than 0.01) when the Singer strain was used in the MDHS system. The SN test with the Singer strain also yielded significantly higher titers for sera from 200 Alberta cattle.
本文描述了一种用于检测牛病毒性腹泻病毒（BVDV）抗体的三日血清中和（SN）试验，该试验是对现有的五日试验的改进。改进后的试验能产生更快的病毒细胞病变效应，并使用马迪达比犬肾（MDBK）细胞以及马血清作为培养基补充剂。对使用BVDV的NADL株和辛格株进行的试验，以及使用二代牛肾细胞加小牛血清（BKCS）或连续MDBK细胞加马血清（MDHS）的情况进行了比较。对来自445头魁北克和安大略省牛的685份血清样本的SN结果分析表明，正如预期的那样，当在BKCS或MDHS系统中使用NADL株时，SN抗体滴度的平均值没有差异，但当在MDHS系统中使用辛格株时，SN抗体滴度升高（p小于0.01）。使用辛格株进行的SN试验对来自200头艾伯塔省牛的血清也产生了显著更高的滴度。
