Deregt D, Masri S A, Cho H J, Bielefeldt Ohmann H
Animal Diseases Research Institute, Lethbridge, Alberta.
Can J Vet Res. 1990 Jun;54(3):343-8.
Monoclonal antibodies reactive to the bovine viral diarrhea virus (BVDV) protein gp53 were produced and characterized. These antibodies and our panel of anti-p80/125 monoclonal antibodies were tested for their cross-reactivity with 11 different North American and European (Danish) BVDV strains and isolates including viruses of both cytopathic and noncytopathic biotypes. The four anti-gp53 monoclonal antibodies were neutralizing for the homologous Danish cytopathic isolate and cross-reacted with all BVDV strains examined except for the Draper strain. Further, anti-gp53 monoclonal antibodies neutralized the majority of BVDV strains examined. The anti-p80/125 monoclonal antibodies cross-reacted with all eleven strains and isolates tested. This indicated that various strains of BVDV have common epitopes. The broad cross-reactivities demonstrated by these monoclonal antibodies suggest that a pool of these antibodies may be used for detection of BVDV cellular contamination or for virus isolation, in place of polyclonal antiserum.
制备并鉴定了对牛病毒性腹泻病毒（BVDV）蛋白gp53具有反应性的单克隆抗体。检测了这些抗体以及我们的抗p80/125单克隆抗体与11种不同的北美和欧洲（丹麦）BVDV毒株及分离株的交叉反应性，这些毒株和分离株包括细胞病变型和非细胞病变型生物型的病毒。这四种抗gp53单克隆抗体对同源的丹麦细胞病变型分离株具有中和作用，并且与除德雷珀毒株外的所有检测BVDV毒株发生交叉反应。此外，抗gp53单克隆抗体中和了大多数检测的BVDV毒株。抗p80/125单克隆抗体与所有测试的11种毒株和分离株发生交叉反应。这表明不同的BVDV毒株具有共同的表位。这些单克隆抗体所显示的广泛交叉反应性表明，这些抗体的混合物可用于检测BVDV细胞污染或用于病毒分离，以替代多克隆抗血清。
