Lipniunas P, Angel A S, Erlansson K, Lindh F, Nilsson B
BioCarb Technology AB, Lund, Sweden.
Anal Biochem. 1992 Jan;200(1):58-67. doi: 10.1016/0003-2697(92)90277-e.
High-mannose oligosaccharides were treated with a mixture of trifluoroacetic acid and trifluoroacetic anhydride under conditions where reducing terminal N-acetylglucosamine residues are specifically degraded. The resulting mannose-containing products were, after further chemical modifications, analyzed by mass spectrometry in fast atom bombardment and electron ionization modes. Binding positions between monosaccharide residues were deduced from mass spectra of peracetylated compounds, which, prior to the derivatization, had been subjected to periodate oxidation and borodeuteride reduction.
