Yamaguchi Y, Hironaka T, Kajiwara M, Tateno E, Kita H, Hirai K
Department of Cell Regulation, Tokyo Medical and Dental University, Japan.
J Virol Methods. 1992 May;37(2):209-18. doi: 10.1016/0166-0934(92)90048-i.
The presence of inhibitors in urine interferes with the enzymatic reaction of the polymerase chain reaction (PCR) for detection of human cytomegalovirus (HCMV). To remove inhibitors, HCMV virions in urine were precipitated with polyethylene glycol, or DNA was extracted from urine by the use of glass powder and subjected to PCR followed by Southern blot hybridization with alkaline phosphatase-linked oligonucleotide probes. These simple, rapid methods increased significantly the sensitivity of PCR for detection of HCMV in urine.
尿液中抑制剂的存在会干扰用于检测人巨细胞病毒（HCMV）的聚合酶链反应（PCR）的酶促反应。为去除抑制剂，用聚乙二醇沉淀尿液中的HCMV病毒粒子，或使用玻璃粉从尿液中提取DNA，然后进行PCR，接着用碱性磷酸酶连接的寡核苷酸探针进行Southern印迹杂交。这些简单、快速的方法显著提高了PCR检测尿液中HCMV的灵敏度。
