Sumiyoshi Y, Kikuchi M, Minematu T, Ohshima K, Takeshita M, Minamishima Y
First Department of Pathology, School of Medicine, Fukuoka University, Japan.
Virchows Arch. 1994;424(4):437-40. doi: 10.1007/BF00190567.
We examined the cervical lymph nodes of 30 patients with Kikuchi's disease and 15 patients with non-specific lymphadenitis, using Southern blot analysis and polymerase chain reaction (PCR) to identify human herpesviruses such as Epstein-Barr virus (EBV), cytomegalovirus, herpes simplex virus, and varicella-zoster virus. By Southern blot analysis, no virus DNA was recognized, but 16 of the 30 nodes from patients with Kikuchi's disease and 8 of the 15 nodes from patients with non-specific lymphadenitis showed amplified EBV DNA by PCR.
我们使用Southern印迹分析和聚合酶链反应(PCR)来鉴定人类疱疹病毒,如EB病毒(EBV)、巨细胞病毒、单纯疱疹病毒和水痘带状疱疹病毒,检查了30例菊池病患者和15例非特异性淋巴结炎患者的颈部淋巴结。通过Southern印迹分析,未识别到病毒DNA,但菊池病患者的30个淋巴结中有16个,非特异性淋巴结炎患者的15个淋巴结中有8个通过PCR显示EBV DNA扩增。