Li H D, Li X C, Zhao B Y
Department of Physiology, Third Military Medical College, Chongqing, China.
Zhongguo Yao Li Xue Bao. 1992 Mar;13(2):146-9.
Effects of ACTH on the content of 5-HT in the hippocampus, midbrain-pons, and spinal cord and the concentration of blood glucose in rats by spectrofluorometric assay and glucose oxidase method were studied. ACTH ip 10 IU.kg-1 or 20 IU.kg-1 significantly increased the content of 5-HT in the hippocampus, midbrain-pons and spinal cord and blood glucose level, both in a dose-dependent manner; para-chloroaphetamine, p-Cpa 4 mg icv markedly reduced the 5-HT in these brain regions and spinal cord and the blood glucose level (P less than 0.01); the level of 5-HT and blood glucose were not significantly altered after icv p-Cpa+ip ACTH; adrenalectomy+ip ACTH markedly increased the 5-HT content in the brain regions and spinal cord, but the blood glucose was decreased (P less than 0.01); and 5-HT was markedly decreased by sc alloxan tetrahydrate+ip ACTH, but blood glucose did not decrease (P greater than 0.05). Thus, ACTH may influence the blood glucose level through central nervous system 5-HT, and the change of 5-HT may be related to the insulin (secretion).
采用荧光分光光度法和葡萄糖氧化酶法，研究促肾上腺皮质激素（ACTH）对大鼠海马、中脑-脑桥和脊髓中5-羟色胺（5-HT）含量及血糖浓度的影响。腹腔注射10IU·kg-1或20IU·kg-1的ACTH均能显著增加海马、中脑-脑桥和脊髓中5-HT的含量以及血糖水平，且呈剂量依赖性；脑室内注射4mg对氯苯丙氨酸（p-Cpa）能显著降低这些脑区、脊髓中的5-HT含量以及血糖水平（P<0.01）；脑室内注射p-Cpa后再腹腔注射ACTH，5-HT水平和血糖水平无显著变化；肾上腺切除术后腹腔注射ACTH能显著增加脑区和脊髓中5-HT的含量，但血糖降低（P<0.01）；皮下注射四水合阿脲后腹腔注射ACTH，5-HT显著降低，但血糖未降低（P>0.05）。因此，ACTH可能通过中枢神经系统5-HT影响血糖水平，5-HT的变化可能与胰岛素（分泌）有关。
