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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Naus C C, Zhu D, Todd S D, Kidder G M

Department of Anatomy, University of Western Ontario, London, Canada.

Cell Mol Neurobiol. 1992 Apr;12(2):163-75. doi: 10.1007/BF00713370.

C6 glioma cells transfected with connexin43 cDNA display a dramatic increase in the level of connexin43 mRNA and protein. 2. This overexpression of connexin43 is evident at the cellular level, as revealed with in situ hybridization and immunocytochemistry. Transfection with connexin43 cDNA also induced actin stress fibers in these glioma cells. 3. Although we observed up to a 50-fold increase in the level of connexin43 mRNA following transfection, virtually all of this mRNA was present in the polysomal fraction. 4. Overexpression of connexin43 mRNA did not appear to compete with other cellular mRNAs for access to the translational machinery. 5. It is likely that the reduced proliferation rate of the transfected cells, reported earlier, is due to enhanced connexin43 expression and intercellular coupling.

用连接蛋白43 cDNA转染的C6胶质瘤细胞，其连接蛋白43 mRNA和蛋白水平显著增加。2. 如原位杂交和免疫细胞化学所示，连接蛋白43的这种过表达在细胞水平上很明显。用连接蛋白43 cDNA转染还诱导了这些胶质瘤细胞中的肌动蛋白应激纤维。3. 尽管我们观察到转染后连接蛋白43 mRNA水平增加了50倍，但几乎所有这种mRNA都存在于多核糖体部分。4. 连接蛋白43 mRNA的过表达似乎并未与其他细胞mRNA竞争进入翻译机制。5. 先前报道的转染细胞增殖率降低可能是由于连接蛋白43表达增强和细胞间偶联所致。

Naus C C, Zhu D, Todd S D, Kidder G M

Department of Anatomy, University of Western Ontario, London, Canada.

Cell Mol Neurobiol. 1992 Apr;12(2):163-75. doi: 10.1007/BF00713370.

C6 glioma cells transfected with connexin43 cDNA display a dramatic increase in the level of connexin43 mRNA and protein. 2. This overexpression of connexin43 is evident at the cellular level, as revealed with in situ hybridization and immunocytochemistry. Transfection with connexin43 cDNA also induced actin stress fibers in these glioma cells. 3. Although we observed up to a 50-fold increase in the level of connexin43 mRNA following transfection, virtually all of this mRNA was present in the polysomal fraction. 4. Overexpression of connexin43 mRNA did not appear to compete with other cellular mRNAs for access to the translational machinery. 5. It is likely that the reduced proliferation rate of the transfected cells, reported earlier, is due to enhanced connexin43 expression and intercellular coupling.

用连接蛋白43 cDNA转染的C6胶质瘤细胞，其连接蛋白43 mRNA和蛋白水平显著增加。2. 如原位杂交和免疫细胞化学所示，连接蛋白43的这种过表达在细胞水平上很明显。用连接蛋白43 cDNA转染还诱导了这些胶质瘤细胞中的肌动蛋白应激纤维。3. 尽管我们观察到转染后连接蛋白43 mRNA水平增加了50倍，但几乎所有这种mRNA都存在于多核糖体部分。4. 连接蛋白43 mRNA的过表达似乎并未与其他细胞mRNA竞争进入翻译机制。5. 先前报道的转染细胞增殖率降低可能是由于连接蛋白43表达增强和细胞间偶联所致。