Yang Y B, Harrison K, Carr D, Guiochon G
Vydac/Separations Group, Hesperia, CA 92345.
J Chromatogr. 1992 Jan 24;590(1):35-47. doi: 10.1016/0021-9673(92)87004-r.
The optimum conditions for the purification of proteins by gradient elution in reversed-phase liquid chromatography were studied, with emphasis on the column length. Because of the strong dependence of the retention of proteins on the mobile phase composition, very short columns can be used successfully to perform analytical separations. A similar conclusion is extended to preparative separations. Columns with different lengths and diameters were used. The dependence of the loading capacity for touching band separation on the column length, diameter and volume was studied, in addition to the regeneration time between successive runs, the starting mobile phase composition and the necessary column efficiency.
研究了反相液相色谱中通过梯度洗脱纯化蛋白质的最佳条件，重点是柱长。由于蛋白质保留对流动相组成的强烈依赖性，非常短的柱子可成功用于进行分析分离。类似的结论也适用于制备分离。使用了不同长度和直径的柱子。除了连续运行之间的再生时间、起始流动相组成和必要的柱效外，还研究了接触带分离的负载量对柱长、直径和体积的依赖性。
