Hamelin C, Lussier G
Virology Research Center, Institut Armand-Frappier, Laval, Qc, Canada.
Lab Anim Sci. 1992 Apr;42(2):142-5.
Restriction endonuclease analysis, dot-blot hybridization, and dried gel hybridization were used to differentiate mouse cytomegalovirus, rat cytomegalovirus, and mouse herpesvirus strain 76. Viral DNA was obtained directly from virus-infected mouse or rat cells. Restriction endonuclease digestion was performed by standard methods with BamHI, EcoRI, HindIII, and PstI, and DNA was analyzed by electrophoresis on agarose gels. Cross-hybridization was used to determine the degree of genetic homology among the three viruses. Electrophoretic patterns revealed clear differences between mouse cytomegalovirus and rat cytomegalovirus restriction profiles. Extensive comigration of DNA was observed for rat cytomegalovirus and mouse herpesvirus strain 76. Both viruses also shared common DNA sequences. These results suggest that mouse herpesvirus strain 76 is probably a rat cytomegalovirus strain infecting mice.
采用限制性内切酶分析、斑点杂交和干凝胶杂交技术来区分小鼠巨细胞病毒、大鼠巨细胞病毒和小鼠疱疹病毒76株。病毒DNA直接从病毒感染的小鼠或大鼠细胞中获得。用BamHI、EcoRI、HindIII和PstI通过标准方法进行限制性内切酶消化，并在琼脂糖凝胶上进行电泳分析DNA。采用交叉杂交来确定这三种病毒之间的基因同源程度。电泳图谱显示小鼠巨细胞病毒和大鼠巨细胞病毒的限制性图谱存在明显差异。观察到大鼠巨细胞病毒和小鼠疱疹病毒76株的DNA有大量共迁移现象。这两种病毒也共享共同的DNA序列。这些结果表明，小鼠疱疹病毒76株可能是一种感染小鼠的大鼠巨细胞病毒株。
