Babinchak T J, Fass R J
Department of Internal Medicine, Ohio State University College of Medicine, Columbus.
Diagn Microbiol Infect Dis. 1992 May-Jun;15(4):367-70. doi: 10.1016/0732-8893(92)90026-p.
The in vitro activities of the DNA gyrase inhibitors ciprofloxacin, coumermycin, and novobiocin against 31 clinical isolates of Mycobacterium avium complex were studied using a microdilution technique. Minimal inhibitory concentrations (MICs) were determined in 4 days using Middlebrook 7H9 broth, and minimal bactericidal concentrations (MBCs) were determined by subculturing to Middlebrook 7H10 agar. MICs were: ciprofloxacin, 0.5-greater than 16 (mean, 4.1) micrograms/ml; novobiocin, 4-greater than 128 (mean, 54.7) micrograms/ml; and coumermycin, 2-greater than 16 (mean, 17.5) micrograms/ml. MBCs were usually more than two dilution steps higher than MICs. Checkerboard studies failed to reveal synergistic or antagonistic inhibitory activity of DNA gyrase-A and DNA gyrase-B inhibitors in vitro.
采用微量稀释技术研究了DNA回旋酶抑制剂环丙沙星、香豆霉素和新生霉素对31株鸟分枝杆菌复合体临床分离株的体外活性。使用Middlebrook 7H9肉汤在4天内测定最低抑菌浓度（MIC），并通过接种到Middlebrook 7H10琼脂上测定最低杀菌浓度（MBC）。MIC分别为：环丙沙星，0.5至大于16（平均4.1）微克/毫升；新生霉素，4至大于128（平均54.7）微克/毫升；香豆霉素，2至大于16（平均17.5）微克/毫升。MBC通常比MIC高两个以上稀释度。棋盘法研究未能揭示DNA回旋酶A和DNA回旋酶B抑制剂在体外的协同或拮抗抑制活性。
