Dutton A, Rees E D, Singer S J
Proc Natl Acad Sci U S A. 1976 May;73(5):1532-6. doi: 10.1073/pnas.73.5.1532.
An experiment has been carried out to test the rotating carrier mechanism of the translocation event in membrane transport. To the Ca-ATPase in intact sarcoplasmic reticulum membranes, 2,4-[3H]dinitrophenyl-cadaverine has been covalently attached by the action of the enzyme, transglutaminase. The binding of anti-2,4-dinitrophenyl antibodies to the 2,4-dinitrophenyl-modified membranes was found to have no effect on either the Ca-ATPase activity or the Ca ion transport rate of the membranes. These results rule out the rotating carrier mechanism in this system. A different scheme for the translocation process, the aggregate rearrangement mechanism, is discussed.
已经进行了一项实验来测试膜运输中转位事件的旋转载体机制。通过转谷氨酰胺酶的作用，将2,4-[³H]二硝基苯尸胺共价连接到完整肌浆网膜中的钙ATP酶上。发现抗2,4-二硝基苯抗体与2,4-二硝基苯修饰膜的结合对膜的钙ATP酶活性或钙离子运输速率均无影响。这些结果排除了该系统中的旋转载体机制。文中讨论了转位过程的另一种方案，即聚集体重排机制。
