Thunnissen F B, Peterse J L, Buchholtz R, Van der Beek J M, Bosman F T
Department of Pathology, University Hospital Maastricht, The Netherlands.
Cytopathology. 1992;3(2):101-9. doi: 10.1111/j.1365-2303.1992.tb00032.x.
Occasionally, in fine-needle aspirates of pleomorphic salivary gland adenomas, considerable cytonuclear atypia is present, which may give rise to a false-positive diagnosis. In this study DNA cytophotometry was performed on Feulgen restained smears prepared from material obtained by needle aspirates of normal salivary glands (n = 4), pleomorphic adenomas with (n = 5) and without (n = 4) atypia and a carcinoma in a pleomorphic adenoma. The results showed a clear diploid DNA histogram in the specimens of normal salivary gland and pleomorphic adenomas without atypia. In contrast, in the pleomorphic adenomas with atypia a distinct polyploid pattern was present in three out of the five DNA histograms with DNA values in 2c, 4c and 8c ranges. In two of these cases a 16c peak was also present and in the two remaining cases tetraploidy was demonstrated. In the carcinoma a main stemline at 4c was found. This report once more emphasizes the possible atypia which may be present in FNA of pleomorphic adenomas of the salivary gland. The atypia is due to polyploidy in a histologically benign tumour.
偶尔，在多形性腺瘤的细针穿刺抽吸物中，会出现明显的细胞核异型性，这可能导致假阳性诊断。在本研究中，对正常唾液腺（n = 4）、有异型性（n = 5）和无异型性（n = 4）的多形性腺瘤以及多形性腺瘤中的癌的针吸材料制备的福尔根复染涂片进行了DNA细胞光度测定。结果显示，正常唾液腺和无异型性的多形性腺瘤标本中有清晰的二倍体DNA直方图。相反，在有异型性的多形性腺瘤中，五个DNA直方图中有三个呈现出明显的多倍体模式，DNA值在2c、4c和8c范围内。其中两例还出现了16c峰，其余两例显示为四倍体。在癌中发现了4c的主要干系。本报告再次强调了唾液腺多形性腺瘤细针穿刺抽吸中可能存在的异型性。这种异型性是由于组织学上良性肿瘤中的多倍体所致。
