Brown W J, Sullivan T R, Greenspan P
Department of Pathology, School of Medicine, University of South Carolina, Columbia 29208.
Histochemistry. 1992 May;97(4):349-54. doi: 10.1007/BF00270037.
We have employed the fluorescent dye nile red to distinguish between normal cells and cells containing lysosomal accumulations of phospholipids. When fibroblasts from an individual with a genetic deficiency in lysosomal sphingomyelinase activity (Niemann-Pick disease) were stained with nile red and visualized by fluorescence microscopy, orange-colored inclusions were observed throughout the cytoplasm. The orange fluorescent bodies could be distinguished from the neutral lipid droplets that fluoresce a brilliant yellow-gold in the presence of nile red. These inclusions were also observed in alveolar macrophages obtained from rats treated with amiodarone, an antiarrhythmic agent known to produce lysosomal phospholipidosis. Flow cytofluorometric analysis revealed that staining of these phospholipid-rich macrophages with nile red can distinguish them from control alveolar macrophages. These results demonstrate that nile red can be employed for the rapid staining of cellular phospholipid inclusions.
我们使用了荧光染料尼罗红来区分正常细胞和含有溶酶体磷脂蓄积的细胞。当用尼罗红对来自溶酶体鞘磷脂酶活性存在基因缺陷个体（尼曼-匹克病）的成纤维细胞进行染色，并通过荧光显微镜观察时，在整个细胞质中都观察到了橙色的内含物。橙色荧光小体可以与在尼罗红存在下发出亮黄-金色荧光的中性脂滴区分开来。在用抗心律失常药物胺碘酮处理的大鼠的肺泡巨噬细胞中也观察到了这些内含物，已知胺碘酮会导致溶酶体磷脂沉积症。流式细胞荧光分析显示，用尼罗红对这些富含磷脂的巨噬细胞进行染色可以将它们与对照肺泡巨噬细胞区分开来。这些结果表明，尼罗红可用于细胞磷脂内含物的快速染色。
