血管紧张素I的纯化
The purification of hypertensin I.
作者信息
SKEGGS L T, MARSH W H, KAHN J R, SHUMWAY N P
出版信息
J Exp Med. 1954 Oct 1;100(4):363-70. doi: 10.1084/jem.100.4.363.
Abstract
The purification of hypertensin I has been described. The final product which is four times as powerful a pressor agent as l-arterenol, is obtained with an over-all recovery of 40 per cent. The product consists of a single component in countercurrent distribution, having a nitrogen content of 15.97 per cent and a specific activity of 7050 Goldblatt units per mg. of N or 1125 units per mg. of solid. Acid hydrolysis and paper chromatography indicate in a preliminary fashion that there are about nine amino acids present in the intact polypeptide.
摘要
高血压素I的提纯方法已有报道。最终产物作为一种升压剂，其效力是去甲肾上腺素的四倍，总回收率为40%。该产物在逆流分配中为单一成分，含氮量为15.97%，比活性为每毫克氮7050戈德布拉特单位或每毫克固体1125单位。酸水解和纸层析初步表明，完整的多肽中约含有九种氨基酸。
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2
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3
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