血管紧张素转换酶的制备与功能。
The preparation and function of the hypertensin-converting enzyme.
作者信息
SKEGGS L T, KAHN J R, SHUMWAY N P
出版信息
J Exp Med. 1956 Mar 1;103(3):295-9. doi: 10.1084/jem.103.3.295.
Abstract
It has been shown by use of isolated, perfused rat kidneys that hypertensin II is a potent vasoconstrictor substance while hypertensin I is not. Hence it would appear that in intact animals the pressor activity of hypertensin I results from its rapid conversion to hypertensin II. An enzyme which effects this conversion has been procured from horse plasma in a semipurified form by means of ammonium sulfate fractionation and isoelectric precipitation. A method is described for estimating the activity of the enzyme. An example of the use of the preparation in converting purified hypertensin I to hypertensin II has been described.
摘要
利用离体灌注大鼠肾脏的实验表明，血管紧张素II是一种强效血管收缩物质，而血管紧张素I则不是。因此，在完整动物体内，血管紧张素I的升压活性似乎源于其迅速转化为血管紧张素II。通过硫酸铵分级分离和等电沉淀法，已从马血浆中以半纯化形式获得了一种能实现这种转化的酶。本文描述了一种估算该酶活性的方法。还介绍了该制剂用于将纯化的血管紧张素I转化为血管紧张素II的一个实例。
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本文引用的文献
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Artificial kidney; construction and operation of an improved continuous dialyzer.人工肾；一种改进型连续透析器的构造与操作
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Amino acid composition and electrophoretic properties of hypertensin I.血管紧张素I的氨基酸组成和电泳特性。
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The purification of hypertensin I.血管紧张素I的纯化
J Exp Med. 1954 Oct 1;100(4):363-70. doi: 10.1084/jem.100.4.363.
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The existence of two forms of hypertensin.高血压素两种形式的存在。
J Exp Med. 1954 Mar;99(3):275-82. doi: 10.1084/jem.99.3.275.
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A method of assaying small amounts of hypertensin.一种检测少量高血压素的方法。
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