Ruiz-Grande C, Alarcón C, Mérida E, Valverde I
Department of Metabolismo, Fundación Jiménez Díaz, Madrid, Spain.
Peptides. 1992 Jan-Feb;13(1):13-6. doi: 10.1016/0196-9781(92)90134-o.
The lipolytic effect of GLP-1(1-36)-amide, GLP-1(7-36) amide and GLP-2 [proglucagon(126-159)] has been studied in isolated rat adipocytes. Glycerol release and cyclic AMP content were measured after incubation of adipocytes with GLPs and results have been compared with those obtained in the presence of glucagon. GLP-1(7-36)-amide and GLP-1(1-36)-amide at 10(-8), 10(-7) and 10(-6) M concentrations activated glycerol release, the truncated peptide having a more potent effect. On the other hand, GLP-2 had no effect on glycerol release. Also, it has been found that 10(-6) M GLP-1(7-36)-amide increases cyclic AMP content in adipocytes and does not compete with glucagon binding. These results demonstrate that GLP-1(7-36)-amide has a lipolytic effect on isolated rat adipocytes through different receptors than glucagon.
已在分离的大鼠脂肪细胞中研究了GLP-1(1-36)-酰胺、GLP-1(7-36)酰胺和GLP-2[胰高血糖素原(126-159)]的脂解作用。在用GLP孵育脂肪细胞后测量甘油释放量和环磷酸腺苷含量，并将结果与在胰高血糖素存在下获得的结果进行比较。浓度为10(-8)、10(-7)和10(-6)M的GLP-1(7-36)-酰胺和GLP-1(1-36)-酰胺可激活甘油释放，截短的肽具有更强的作用。另一方面，GLP-2对甘油释放无影响。此外，还发现10(-6)M的GLP-1(7-36)-酰胺可增加脂肪细胞中环磷酸腺苷的含量，且不与胰高血糖素结合竞争。这些结果表明，GLP-1(7-36)-酰胺对分离的大鼠脂肪细胞具有脂解作用，其作用途径与胰高血糖素不同，通过不同的受体发挥作用。
