尿中NO2-/NO3-排泄增加作为血压升高期间内皮源性舒张因子释放的指标。

Increases in NO2-/NO3- excretion in the urine as an indicator of the release of endothelium-derived relaxing factor during elevation of blood pressure.

Suzuki H, Ikenaga H, Hishikawa K, Nakaki T, Kato R, Saruta T

Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.

Clin Sci (Lond). 1992 Jun;82(6):631-4. doi: 10.1042/cs0820631.

Abstract

Under hormonally constant conditions, the effects of a sudden increase in blood pressure on the release of endothelium-derived relaxing factor were evaluated by measuring urinary excretion of NO2-/NO3- in rats with renal denervation. 2. Elevation of blood pressure from 136 +/- 2 to 153 +/- 3 mmHg by an aortic clamp below the renal arteries induced a significant increase in urinary excretion of NO2-/NO3- from 76.6 +/- 4.2 x 10(2) to 108.1 +/- 8.3 x 10(2) pmol min-1 g-1 kidney weight (P less than 0.05). 3. Infusion of NG-monomethyl-L-arginine (1 mg min-1 kg-1) without an aortic clamp raised mean blood pressure to a similar level; however, urinary excretion of NO2-/NO3- was decreased significantly. 4. During infusion of NG-monomethyl-L-arginine, aortic occlusion caused a significant increase in blood pressure without any changes in NO2-/NO3- excretion in the urine. 5. These results suggest that the formation of NO, an indicator of endothelium-derived relaxing factor release, was increased by mechanical pressure elevation without apparent changes in hormonal and neural factors.

摘要

在激素水平恒定的条件下，通过测量去肾神经大鼠尿中NO2-/NO3-的排泄量，评估血压突然升高对内皮衍生舒张因子释放的影响。2. 用肾动脉下方的主动脉夹将血压从136±2 mmHg升高至153±3 mmHg，可使尿中NO2-/NO3-的排泄量从76.6±4.2×10(2)显著增加至108.1±8.3×10(2) pmol min-1 g-1肾重（P<0.05）。3. 在未使用主动脉夹的情况下输注NG-单甲基-L-精氨酸（1 mg min-1 kg-1）可使平均血压升高至相似水平；然而，尿中NO2-/NO3-的排泄量显著降低。4. 在输注NG-单甲基-L-精氨酸期间，主动脉阻断导致血压显著升高，而尿中NO2-/NO3-的排泄量无任何变化。5. 这些结果表明，作为内皮衍生舒张因子释放指标的NO的生成，在激素和神经因素无明显变化的情况下，因机械性压力升高而增加。