Hughes P, Lawlor P, Dragunow M
Department of Pharmacology and Clinical Pharmacology, University of Auckland School of Medicine, Private Bag, New Zealand.
Brain Res Mol Brain Res. 1992 May;13(4):355-7. doi: 10.1016/0169-328x(92)90219-2.
The basal expression of the protein products of the inducible immediate early genes (IEGs), Fos, Jun, and Krox 24, was investigated in rat hippocampus using immunocytochemical visualization methods with antisera specific for Fos only, Fos and the Fos-related antigens (FRAs), the Jun family, and Krox 24 (previously described as TIS 8, egr-1, NGF-IA or zif 268). In the normal adult rat brain basal levels of Jun, Krox 24 and Fos-related antigens but not Fos were seen within the hippocampus. More specifically very high basal levels of Jun were seen in the dentate granule cells with high basal Krox 24 levels seen in the CA1-subiculum region of the rat hippocampus. Basal FRAs but not Fos-positive cells were seen at low levels in the dentate granule cells. The implications of these results to the functioning of IEG proteins in hippocampal neurons is discussed.
利用仅针对Fos、Fos和Fos相关抗原（FRA）、Jun家族以及Krox 24（先前称为TIS 8、egr-1、NGF-IA或zif 268）的抗血清免疫细胞化学可视化方法，研究了诱导型即刻早期基因（IEG）的蛋白质产物Fos、Jun和Krox 24在大鼠海马体中的基础表达。在正常成年大鼠大脑中，海马体内可检测到Jun、Krox 24和Fos相关抗原的基础水平，但未检测到Fos的基础水平。更具体地说，在齿状颗粒细胞中可见非常高的Jun基础水平，在大鼠海马体的CA1-海马下区域可见高基础水平的Krox 24。在齿状颗粒细胞中可低水平观察到基础FRA阳性细胞，但未观察到Fos阳性细胞。讨论了这些结果对海马神经元中IEG蛋白功能的影响。
