Bacon L D, Witter R L
Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
Avian Dis. 1992 Apr-Jun;36(2):378-85.
Eight recently developed 15.B congenic lines of chickens were tested for Marek's disease (MD) resistance by intra-abdominal injection of cell-associated preparations of MD virus of a virulent strain (JM), a very virulent strain (Md5), or Md5 after vaccination with turkey herpesvirus (HVT) strain FC126. Chickens of the 15.N congenic line (B15B21 or B21B21) were very resistant to JM-induced MD, in contrast to chickens homozygous for the B-haplotypes 2, 5, 12, 13, 15, or 19. After Md5 infection, more than 88% of the chickens in all of the congenic lines developed MD. However, when chickens were vaccinated with HVT before being inoculated with Md5, the B5 and B12 homozygotes were more resistant to MD than were the B2, B13, or B19 homozygotes, and B15 and B21 homozygotes had intermediate resistance. B5B5 and B2B5 F2 chicks inoculated with HVT and Md5 had a lower prevalence of MD than B2B2 sibs. These results demonstrate that a protocol involving HVT vaccination of chicks followed by infection with very virulent MD virus will allow the detection of B-haplotypes determining MD resistance, some of which are not detectable in unvaccinated chicks challenged with virulent MD.
通过腹腔注射强毒株（JM）、超强毒株（Md5）的马立克氏病病毒（MDV）细胞相关制剂或用火鸡疱疹病毒（HVT）FC126株疫苗接种后再注射Md5，对最近培育出的8个15.B同源鸡系进行马立克氏病（MD）抗性测试。与B单倍型2、5、12、13、15或19的纯合鸡相比，15.N同源鸡系（B15B21或B21B21）的鸡对JM诱导的MD具有很强的抗性。在感染Md5后，所有同源鸡系中超过88%的鸡发生了MD。然而，当鸡在用Md5接种前先用HVT进行疫苗接种时，B5和B12纯合子比B2、B13或B19纯合子对MD更具抗性，B15和B21纯合子具有中等抗性。接种HVT和Md5的B5B5和B2B5 F2雏鸡的MD患病率低于B2B2同胞。这些结果表明，一种涉及雏鸡接种HVT然后感染超强毒力MD病毒的方案将能够检测出决定MD抗性的B单倍型，其中一些在接种强毒力MD的未接种疫苗雏鸡中无法检测到。
