一种基于多态性序列标签位点的秀丽隐杆线虫基因图谱系统。
A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.
作者信息
Williams B D, Schrank B, Huynh C, Shownkeen R, Waterston R H
机构信息
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
Genetics. 1992 Jul;131(3):609-24. doi: 10.1093/genetics/131.3.609.
Abstract
We devised an efficient genetic mapping system in the nematode Caenorhabditis elegans which is based upon the differences in number and location of the transposable element Tc1 between the Bristol and Bergerac strains. Using the nearly completed physical map of the C. elegans genome, we selected 40 widely distributed sites which contain a Tc1 element in the Bergerac strain, but not in the Bristol strain. For each site a polymerase chain reaction assay was designed that can distinguish between the Bergerac Tc1-containing site and the Bristol "empty" site. By combining appropriate assays in a single reaction, one can score multiple sites within single worms. This permits a mutation to be rapidly mapped, first to a linkage group and then to a chromosomal subregion, through analysis of only a small number of progeny from a single interstrain cross.
摘要
我们在秀丽隐杆线虫中设计了一种高效的遗传图谱系统，该系统基于布里斯托尔菌株和伯杰拉克菌株之间转座元件Tc1数量和位置的差异。利用近乎完整的秀丽隐杆线虫基因组物理图谱，我们选择了40个分布广泛的位点，这些位点在伯杰拉克菌株中含有一个Tc1元件，但在布里斯托尔菌株中没有。针对每个位点设计了一种聚合酶链反应检测方法，该方法可以区分含有伯杰拉克Tc1的位点和布里斯托尔的“空”位点。通过在单个反应中组合适当的检测方法，可以对单个蠕虫内的多个位点进行评分。这使得通过分析来自单个品系间杂交的少量后代，能够快速将突变定位到一个连锁群，然后再定位到一个染色体亚区域。
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