神经氨酸酶处理禽传染性支气管炎冠状病毒后显示出一种血凝活性，该活性依赖于红细胞上含唾液酸的受体。
Neuraminidase treatment of avian infectious bronchitis coronavirus reveals a hemagglutinating activity that is dependent on sialic acid-containing receptors on erythrocytes.
作者信息
Schultze B, Cavanagh D, Herrler G
机构信息
Institut für Virologie, Philipps-Universität Marburg, Germany.
出版信息
Virology. 1992 Aug;189(2):792-4. doi: 10.1016/0042-6822(92)90608-r.
Abstract
The interaction of infectious bronchitis virus (IBV) with erythrocytes was analyzed. The binding activity of IBV was not sufficient to agglutinate chicken erythrocytes. However, it acquired hemagglutinating activity after treatment with neuraminidase to remove alpha 2,3-linked N-acetylneuraminic acid from the surface of the virion. Pretreatment of erythrocytes with neuraminidase rendered the cells resistant to agglutination by IBV. Susceptibility to agglutination was restored by resialylation of asialo-erythrocytes to contain alpha 2,3-linked sialic acid. These results indicate that IBV attaches to receptors on erythrocytes, the crucial determinant of which is sialic acid alpha 2,3-linked to galactose. In contrast to other enveloped viruses with such a binding specificity (influenza viruses and paramyxoviruses) IBV lacks a receptor-destroying enzyme.
摘要
分析了传染性支气管炎病毒（IBV）与红细胞的相互作用。IBV的结合活性不足以凝集鸡红细胞。然而，在用神经氨酸酶处理以从病毒粒子表面去除α2,3 - 连接的N - 乙酰神经氨酸后，它获得了血凝活性。用神经氨酸酶预处理红细胞使细胞对IBV的凝集具有抗性。通过将去唾液酸红细胞重新唾液酸化以含有α2,3 - 连接的唾液酸，恢复了对凝集的敏感性。这些结果表明，IBV附着于红细胞上的受体，其关键决定因素是与半乳糖α2,3 - 连接的唾液酸。与具有这种结合特异性的其他包膜病毒（流感病毒和副粘病毒）不同，IBV缺乏受体破坏酶。
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