Cox I J, Bell J D, Peden C J, Iles R A, Foster C S, Watanapa P, Williamson R C
NMR Unit, Hammersmith Hospital, London, UK.
NMR Biomed. 1992 May-Jun;5(3):114-20. doi: 10.1002/nbm.1940050303.
In vivo 31P magnetic resonance spectroscopy (MRS) was undertaken in 28 healthy adult individuals and 32 patients with hepatic malignancies of varying histology, using chemical shift imaging techniques. The mean peak area ratio (total range) of phosphomonoester (PME) to phosphodiester (PDE) in the health adult group was 0.23 (0.15-0.41). The mean (total range) PME/PDE ratio of the total patient group was 0.68 (0.15-2.38), which was significantly elevated (P less than 0.001) compared to the mean of the healthy adult group. Liver biopsies, obtained at operation, were analysed using high-field in vitro MRS techniques in order to identify the contributions of aqueous-soluble metabolites to the multicomponent PME and PDE in vivo signals. Concentrations of phosphorylethanolamine (PE), phosphorylcholine (PC), glycerophosphorylethanolamine (GPE) and glycerophosphorylcholine (GPC) were measured. The in vitro spectrum of six samples of liver of normal histological appearance all showed a similar pattern of PE, PC, GPE and GPC. The in vitro spectrum of seven liver tumours of differing histology all showed an increase in PE and PC signals and a decrease in GPC and GPE signals. The in vitro results were compared with in vivo findings in five patients. The increase in PME/PDE observed in vivo represented, in part, an increase in PE and PC in the PME region and a decrease in GPE and GPC in the PDE region.
采用化学位移成像技术，对28名健康成年人和32名不同组织学类型的肝脏恶性肿瘤患者进行了体内31P磁共振波谱（MRS）检查。健康成年组磷酸单酯（PME）与磷酸二酯（PDE）的平均峰面积比（总范围）为0.23（0.15 - 0.41）。所有患者组的平均PME/PDE比（总范围）为0.68（0.15 - 2.38），与健康成年组的平均值相比显著升高（P < 0.001）。术中获取肝脏活检组织，使用高场体外MRS技术进行分析，以确定水溶性代谢物对体内多组分PME和PDE信号的贡献。测量了磷酸乙醇胺（PE）、磷酸胆碱（PC）、甘油磷酸乙醇胺（GPE）和甘油磷酸胆碱（GPC）的浓度。6份组织学外观正常的肝脏样本的体外波谱均显示出PE、PC、GPE和GPC的相似模式。7份不同组织学类型的肝脏肿瘤的体外波谱均显示PE和PC信号增加，GPC和GPE信号减少。将体外结果与5名患者的体内结果进行了比较。体内观察到的PME/PDE增加部分代表PME区域中PE和PC增加以及PDE区域中GPE和GPC减少。
