Bito H, Nakamura M, Honda Z, Izumi T, Iwatsubo T, Seyama Y, Ogura A, Kudo Y, Shimizu T
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Neuron. 1992 Aug;9(2):285-94. doi: 10.1016/0896-6273(92)90167-c.
Platelet-activating factor (PAF), an alkylether phospholipid, is produced in the brain when it is subjected to various stimuli. Using a Xenopus oocyte expression system, we obtained evidence for functional PAF receptor mRNA expression in rat brain. The presence of the PAF receptor was confirmed and shown to be quite ubiquitous in the CNS by RNA blot and radioligand binding studies. To investigate the neuronal functions of PAF, intracellular Ca2+ increase elicited by nanomolar PAF application was analyzed in cultured rat hippocampal cells. Fractions of NMDA-responsive cells and non-NMDA-responsive cells were shown to respond to PAF, suggesting a potential role for PAF in the Ca2+ signaling pathway in the hippocampus.
血小板活化因子（PAF）是一种烷基醚磷脂，当大脑受到各种刺激时会在其中产生。利用非洲爪蟾卵母细胞表达系统，我们获得了大鼠大脑中功能性PAF受体mRNA表达的证据。通过RNA印迹和放射性配体结合研究证实了PAF受体的存在，并表明其在中枢神经系统中相当普遍。为了研究PAF的神经元功能，我们分析了在培养的大鼠海马细胞中，纳摩尔浓度的PAF应用所引发的细胞内Ca2+增加情况。结果显示，NMDA反应性细胞和非NMDA反应性细胞部分都对PAF有反应，这表明PAF在海马体的Ca2+信号通路中可能发挥作用。
