Bito H, Honda Z, Nakamura M, Shimizu T
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Eur J Biochem. 1994 Apr 1;221(1):211-8. doi: 10.1111/j.1432-1033.1994.tb18731.x.
The biological functions of platelet-activating factor (PAF) have been extensively studied in the rat. However, the precise structure and distribution of rat PAF receptor has not been reported. To address this question, we isolated a rat PAF-receptor cDNA from a size-fractionated rat spleen cDNA library. The deduced amino acid sequence of the rat PAF receptor showed 80% and 79% identity with guinea pig and human PAF receptors, respectively. Pharmacological properties (ED50, inhibition by WEB2086) of rat PAF receptors expressed in Xenopus oocytes were similar to those for PAF receptors expressed from guinea pig or human cDNAs. Northern blot analysis showed a widespread distribution of PAF-receptor mRNA in almost all organs including spleen, small intestine, kidney, lung, liver and brain. Considerable difference in the PAF-receptor distribution detected among species suggests the existence of a species-specific and tissue-specific regulatory mechanism for PAF-receptor-mRNA expression. Isolation of rat PAF-receptor cDNA should facilitate further analysis of PAF-receptor function and pharmacology in diverse pathophysiological processes.
血小板活化因子（PAF）的生物学功能已在大鼠中得到广泛研究。然而，大鼠PAF受体的精确结构和分布尚未见报道。为解决这一问题，我们从按大小分级的大鼠脾脏cDNA文库中分离出大鼠PAF受体cDNA。推导的大鼠PAF受体氨基酸序列与豚鼠和人PAF受体的一致性分别为80%和79%。在非洲爪蟾卵母细胞中表达的大鼠PAF受体的药理学特性（半数有效剂量、WEB2086的抑制作用）与从豚鼠或人cDNA表达的PAF受体相似。Northern印迹分析表明，PAF受体mRNA在几乎所有器官中广泛分布，包括脾脏、小肠、肾脏、肺、肝脏和大脑。在不同物种中检测到的PAF受体分布存在显著差异，这表明存在PAF受体mRNA表达的物种特异性和组织特异性调节机制。大鼠PAF受体cDNA的分离应有助于进一步分析PAF受体在各种病理生理过程中的功能和药理学特性。
