Soler C, Chardonnet Y, Euvrard S, Chignol M C, Thivolet J
INSERM U 209, Hôpital E.-Herriot, Lyon, France.
Dermatology. 1992;184(4):248-53. doi: 10.1159/000247561.
Transplant recipients are at high risk to develop multiple cutaneous lesions after grafting. The frequency of the potentially oncogenic human papillomavirus (HPV) type 5 DNA was evaluated in cutaneous lesions taken from sun-exposed areas in transplant recipients (92 lesions and 5 samples from normal skin) and compared with a nontransplanted population (22 lesions and 7 samples from normal skin) using in situ hybridization and biotinylated probes to HPV types 1, 2, 5, 16 and 18. HPV type 5 DNA was identified in 8/92 cutaneous lesions of transplanted recipients: 3 warts, 1 case of seborrheic keratosis, 2 actinic keratoses and 2 keratoacanthomas. HPV type 5 DNA was not detected in 27 malignant tumors (8 basal cell carcinomas and 19 squamous cell carcinomas) from transplant recipients. HPV DNA type 5 was detected in only 1 case of squamous cell carcinoma from the general population. The presence of HPV DNA 5 was confirmed with Southern blotting in 2 out of 6 cases from transplant recipients. The reaction was negative with the squamous cell carcinoma from nontransplant recipients. These data indicate that the presence of HPV DNA type 5 is not very frequent; it can be detected with in situ hybridization and nonisotopic probe, which is easier to handle than Southern blot.
移植受者在移植后发生多发性皮肤病变的风险很高。使用针对1、2、5、16和18型人乳头瘤病毒（HPV）的原位杂交和生物素化探针，对移植受者阳光暴露部位的皮肤病变（92个病变和5个正常皮肤样本）中潜在致癌的5型HPV DNA频率进行了评估，并与非移植人群（22个病变和7个正常皮肤样本）进行了比较。在移植受者的8/92个皮肤病变中鉴定出5型HPV DNA：3个疣、1例脂溢性角化病、2例光化性角化病和2例角化棘皮瘤。在移植受者的27个恶性肿瘤（8个基底细胞癌和19个鳞状细胞癌）中未检测到5型HPV DNA。在普通人群的鳞状细胞癌中仅1例检测到5型HPV DNA。通过Southern印迹法在移植受者的6例中的2例中证实了5型HPV DNA的存在。非移植受者的鳞状细胞癌反应为阴性。这些数据表明，5型HPV DNA的存在并不常见；可以通过原位杂交和非同位素探针检测到，这比Southern印迹法更易于操作。
